Linzhao Cheng, Ph.D.Image

Associate Professor of Stem Cell Program in the Institute of Cell Engineering

Contact Information

Room 747, Broadway Research Building
410-614-6958
443-287-5611 (Fax)
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Link to Institute of Cell Engineering Faculty Page

Link to Dr. Linzhao Cheng's Lab Page


Research Interests

Dr. Linzhao Cheng is a founding member of Stem Cell Program in the Johns Hopkins Institute for Cell Engineering (ICE).  He is also a faculty member of the Graduate Training Programs in Cellular and Molecular Medicine and Immunology.

After receiving a PhD degree in Molecular Biology and Genetics from Hopkins in 1991, Dr. Cheng has been working on stem cell research in NIH, the biotech industry and academia.  He has extensive experience in several types of stem cells including human hematopoietic stem cells (HSC), mesenchymal stem cells/marrow stromal cells (MSC), embryonic stem (ES) cells and induced pluripotent stem (iPS) cells.   In 2003 and nominated by NIH, Dr. Cheng received the Presidential Early Career Award for Scientists and Engineers, "for outstanding accomplishments in the field of stem cell research including pioneering research that is advancing our knowledge of human embryonic stem cell self-renewal and differentiation of blood cells".  This Presidential Award is the highest honor bestowed by the U.S. government on outstanding scientists and engineers beginning their independent careers.

Dr. Cheng’s ICE laboratory is working on human stem cell biology and engineering, and their applications in regenerative medicine.  One of objectives is to understand genetic and epigenetic regulation of cell fate determination.  His group currently focuses on using human pluripotent stem such as iPS cells from healthy donor and patients, including those with targeted or corrected mutations, to investigate human stem cell biology and diseases.  See his publications since he joined ICE in 2003, or visit his lab website: www.stemcelllab.org

Selected Publications

  • Cheng L, Hammond H, Ye Z, Zhan X, and Dravid G (2003). Human adult marrow cells support prolonged expansion of human embryonic stem cells in culture. Stem Cells, 21:131-142.
  • Yu X, Zhan X, D’Costa J, Tanavde VM, Ye Z, Peng T, Malehorn MT, Yang X, Civin CI and Cheng L (2003). Lentiviral Vectors with Two Independent Internal Promoters Transfer High-Level Expression of Multiple Transgenes to Human Hematopoietic Stem-Progenitor Cells.  Molecular Therapy, 7(6): 827-838.
  • Zhou X, Cui Y, Huang X, Yu Z, Thomas AM, Ye Z, Pardoll DM, Jaffee EM, and Cheng L (2003). Lentivirus-Mediated Gene Transfer and Expression in Established Human Cytotoxic T cells Specific to Tumor Antigen and in Primary Unstimulated T Cells. Human Gene Therapy, 14:1089-1105.
  • Pan F, Ye Z, Cheng L, Liu JO (2004). Myocyte enhancer factor 2 mediates calcium-dependent transcription of the interleukin-2 gene in T lymphocytes. J Biol Chem. 279: 14477-14480.
  • Zhan X, Dravid G, Ye Z, Hammond H, Shamblott M, Gearhart J, and Cheng L (2004). Functional antigen-presenting leukocytes derived from human embryonic stem cells: implications for improving transplant outcome. Lancet, 364: 163-171.
  • Dravid G, Ye Z, Hammond H, Chen G, Pyle A, Donovan PJ, Yu X and Cheng L (2005). Defining the role of Wnt/beta-catenin signaling in the survival, proliferation and self-renewal of human embryonic stem cells.  Stem Cells, 23: 1489-1488.
  • Dravid G, Hammond H and Cheng L (2006).  Culture of human embryonic stem cells on human and mouse feeder cells.  In Human embryonic stem cells, Methods in Molecular Biology 331:91-104.
  • Yu, X, Alder JK, Chun JH, Friedman AD, Heimfeld S, Cheng L, Civin CI (2006). HES1 inhibits cycling of hematopoietic progenitor cells via DNA-binding. Stem Cells, 24: 876-888.
  • Cheng, L, Qiu RZ, Deng H, Zhang YA, Jin Y, and Li L (2006). Ethics: China already has clear stem-cell guidelines. Nature, 440:992. (correspondence)
  • Mali P and Cheng L (2006). Re-programming somatic cells without fusion or ethical confusion.  Regenerative Medicine (UK), 1(6): 837-840.
  • Cai L, Ye, Z, Zhou, BY, Mali P, Zhou C and Cheng L (2007).  Promoting human embryonic stem cell renewal or differentiation by modulating Wnt signal. Cell Res, 17: 62-72.
  • Zhou BY, Ye Z, Gao PZ, Chen G, Zhang YA and Cheng L (2007). Inducible and reversible transgene expression in human stem cells after efficient and stable gene transfer. Stem Cells, 25: 779-789.
  • Ohm JE, McGarvey KM, Yu X, Cheng L, Schuebel KE, Cope L, Mohammad HP, Chen W, Daniel VC, Yu W, Berman DM, Jenuwein T, Pruitt K, Sharkis SJ, Watkins DN, Herman JG, Baylin SB (2007). A stem cell-like chromatin pattern may predispose tumor suppressor genes to DNA hepermethylation and silencing in adult cancers. Nature Genetics, 39(2): 237-242.
  • Gao P, Zhang H, Dinavahi R, Li F, Xiang Y, Raman V, Bhujwalla ZM, Felsher DW, Cheng L, Pevsner J, Lee LA, Semenza GL, Dang CV (2007).  HIF-dependent Anti-tumorigenic Effect of Anti-oxidants In Vivo. Cancer Cell, 12(3): 230-238.
  • Ye Z, Yu X, and Cheng L (2007). Lentiviral transduction of human and mouse stem cells. Methods in Molecular Biology 430:243-253.
  • Cheng L, Xiao L, Zeng F, and Zhang YA (2008). Stem cells shine in Shanghai. Cell Stem Cell 2(1):34-37. PMID: 18371419.
  • Cheng L (2008). More new lines of human parthenogenetic embryonic stem cells. Cell Res. 18: 215-217 (Invited mini-review). PMID: 18246085.
  • Chen G, Ye Z, Yu X, Zou J, Mali P, Brodsky RA and Cheng L (2008). Trophoblast differentiation defect in human embryonic stem cells lacking PIG-A and GPI-anchored cell surface proteins. Cell Stem Cell, 2(4): 345-355. PMID: 18397754.
  • Yu X, Zou J, Ye Z, Hammond HH, Chen G, Tokunaga A, Mali P, Li YM, Civin CI, Gaiano N, Cheng L (2008). Notch signaling activation in human embryonic stem cells is required for embryonic but not trophoblastic lineage commitment. Cell Stem Cell, 2(5): 461-471. PMID: 18462696.
  • Mali P, Ye Z, Hammond H, Yu X, Lin J, Chen G, Zou J, Cheng L (2008). Improved efficiency and pace of generating induced pluripotent stem cells from human adult and fetal fibroblasts Stem Cells, 26(8): 1998-2005. PMID: 18511599.
  • Wu Z, Zhang W, Chen G, Cheng L, Liao J, Jia N, Gao Y, Dai H, Yuan J, Cheng L, Xiao L (2008). Combinatorial signals of activin/nodal and bone morphogenic protein regulate the early lineage segregation of human embryonic stem cells. J Biol Chem. 283: 24991. PMID: 18596037.
  • Hillion J, Dhara S, Sumter T, Mukherjee M, Di Cello F, Belton A, Turkson J, Jaganathan S, Cheng L, Ye Z, Jove R, Aplan P, Lin Y-W, Wertzler K, Reeves R, Elbahlouh O, Kowalski J, Bhattacharya R, Resar L (2008). The HMGA1a-STAT3 axis: an "Achilles heel" for hematopoietic malignancies? Cancer Research, 68(24):10121. PMID: 19074878.
  • Hwang NS, Varghese S, Lee HJ, Zhang Z, Bae J, Ye Z, Cheng L, Elisseeff J (2008). In Vivo Commitment and Functional Tissue Regeneration Using Human Embryonic Stem Cell-Derived Mesenchymal Cells. PNAS, 105(52):20641-6. PMID: 19095799.
  • Savage WJ, Barber JP, Mukhina GL, Hu R, Chen G, Matsui W, Thoburn C, Hess AD, Cheng L, Jones RJ, and Brodsky RA (2009).  Glycosylphosphatidylinositol-anchored Protein Deficiency Confers Resistance to Apoptosis in PNH. Experimental Hematology, 37(1):42-51. PMID: 19013003.
  • Pomper MG, Hammond HH, Yu X, Ye Z, Foss CA, Lin DD, Fox JJ, Cheng L (2009). Serial Imaging of Human Embryonic Stem Cell Engraftment and Teratoma Formation in Live Mouse Models. Cell Res. 19(3):370-9. PMID: 19114988.
  • Wang YL and Cheng L (2009). Regulation of primate trophoblast lineage differentiation -- Insights learned from human embryonic stem cells.  Reproductive Biology Insight, accepted.
  • Zou J, Maeder ML, Mali P, Pruett-Miller SM, Thibodeau-Beganny S, Chou BK, Chen G, Ye Z, Park IH, Daley GQ, Porteus MH, Joung JK, Cheng L (2009). Gene targeting of a disease-related gene in human induced pluripotent stem cells and embryonic stem cells. Cell Stem Cell. 5(1):97-110. PMID: 19540188.
  • Cheng L (2009). DNA transposons for stem cells. Blood, 114(7):1285-1286. (Commentary).
  • Ye Z, Zhan H, Mali P, Dowey S, Jang Y-Y, Dang CV, Spivak JL, Moliterno AR, and Cheng L. Human induced pluripotent stem cells from blood cells of healthy donors and patients with acquired blood disorders.  In press, Blood.
 
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